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When you observe fluorescence-labeled specimens in 3D, specimen details outside the depth-of-field of the objective appear blurred. This reduces the signal-to-noise ratio in your data. ApoTome.2 solves this problem by structured illumination.
ApoTome.2 is a module with integrated grid structures. Insert the module into your microscope and leave AxioVision to do the rest for you. Taking account of the objective used, AxioVision automatically places the optimal grid into the microscope's beam path – no manual change is required.
As an excitation illuminant, use the Colibri.2 LED light source or any conventional white light source. Contrary to confocal systems laser excitation is not needed. ApoTome.2 works with, and can be retrofitted, to all partially motorized Axio Imager and Axio Observer microscopes.
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