Superresolution microscopy enables fluorescence imaging of structures too small for traditional methods, such as deconvolution or confocal microscopy. Carl Zeiss has integrated two methods of superresolution into one, turn-key platform. The ELYRA system can be configured for superresolution structured illumination (SR-SIM) and/or photoactivated localization microscopy (PAL-M). It can also be combined with our laser scanning microscopes, the LSM 780 or LSM 710.
The ELYRA S.1, which incorporates SR-SIM technology, is the most flexible superresolution system available. It works with all conventional fluorescent proteins and dyes. Almost any sample that is generated for fluorescent microscopy can be imaged with SR-SIM. By using a precise spatial modulation of the excitation light with an algorithm that computes superresolution information from interference patterns in the raw data, SR-SIM delivers double the resolution in XY and Z compared to deconvolution or confocal microscopy.
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